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Abstract

The identification of human inflammatory cells that express inducible nitric oxide synthase and the clarification of the role of inducible nitric oxide synthase in human infectious or inflammatory processes have been elusive. In neutrophil-enriched fractions from urine, we demonstrate a 43-fold increase in nitric oxide synthase activity in patients with urinary tract infections compared with that in neutrophil-enriched fractions from noninfected controls. Partially purified inducible nitric oxide synthase is primarily membrane associated, calcium independent, and inhibited by arginine analogues with a rank order consistent with that of purified human inducible nitric oxide synthase. Molecular, biochemical, and immunocytochemical evidence unequivocally identifies inducible nitric oxide synthase as the major nitric oxide synthase isoform found in neutrophils isolated from urine during urinary tract infections. Elevated inducible nitric oxide synthase activity and elevated nitric oxide synthase protein measured in patients with urinary tract infections and treated with antibiotics does not decrease until 6-10 d of antibiotic treatment. The extended elevation of neutrophil inducible nitric oxide synthase during urinary tract infections may have both antimicrobial and proinflammatory functions.

Authors

M A Wheeler, S D Smith, G García-Cardeña, C F Nathan, R M Weiss, W C Sessa

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Citations to this article in year 2011 (5)

Title and authors Publication Year
Dynamin2- and endothelial nitric oxide synthase-regulated invasion of bladder epithelial cells by uropathogenic Escherichia coli
Z Wang, C Humphrey, N Frilot, G Wang, Z Nie, NH Moniri, Y Daaka
The Journal of Cell Biology 2011
A calcium-insensitive attenuated nitrosative stress response contributes significantly in the radioresistance of Sf9 insect cells
S Suman, RK Seth, S Chandna
The International Journal of Biochemistry & Cell Biology 2011
Neutrophil extracellular traps contain mitochondrial as well as nuclear DNA and exhibit inflammatory potential
RS Keshari, A Jyoti, S Kumar, M Dubey, A Verma, BS Srinag, H Krishnamurthy, MK Barthwal, M Dikshit
Cytometry Part A 2011
Regulation of Inducible Nitric-oxide Synthase by the SPRY Domain- and SOCS Box-containing Proteins*
T Nishiya, K Matsumoto, S Maekawa, E Kajita, T Horinouchi, M Fujimuro, K Ogasawara, T Uehara, S Miwa
The Journal of biological chemistry 2011
Regulation of T Cell Priming by Lymphoid Stroma
O Khan, M Headley, A Gerard, W Wei, L Liu, MF Krummel
PloS one 2011

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