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Abstract

The identification of human inflammatory cells that express inducible nitric oxide synthase and the clarification of the role of inducible nitric oxide synthase in human infectious or inflammatory processes have been elusive. In neutrophil-enriched fractions from urine, we demonstrate a 43-fold increase in nitric oxide synthase activity in patients with urinary tract infections compared with that in neutrophil-enriched fractions from noninfected controls. Partially purified inducible nitric oxide synthase is primarily membrane associated, calcium independent, and inhibited by arginine analogues with a rank order consistent with that of purified human inducible nitric oxide synthase. Molecular, biochemical, and immunocytochemical evidence unequivocally identifies inducible nitric oxide synthase as the major nitric oxide synthase isoform found in neutrophils isolated from urine during urinary tract infections. Elevated inducible nitric oxide synthase activity and elevated nitric oxide synthase protein measured in patients with urinary tract infections and treated with antibiotics does not decrease until 6-10 d of antibiotic treatment. The extended elevation of neutrophil inducible nitric oxide synthase during urinary tract infections may have both antimicrobial and proinflammatory functions.

Authors

M A Wheeler, S D Smith, G García-Cardeña, C F Nathan, R M Weiss, W C Sessa

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Citations to this article in year 2002 (12)

Title and authors Publication Year
Human renal epithelial cells express iNOS in response to cytokines but not bacteria
M Poljakovic, D Karpman, C Svanborg, K Persson
Kidney International 2002
Inducible nitric oxide synthase is expressed in synovial fluid granulocytes
J Cedergren, T Forslund, T Sundqvist, T Skogh
Clinical & Experimental Immunology 2002
Nitric oxide production by neutrophils obtained from patients during acute coronary syndromes: expression of the nitric oxide synthase isoforms
LS de Miguel, MM Arriero, M Montón, A López-Farré, J Farré, F Cabestrero, E Martín, J Romero, P Jiménez, A García-Méndez, T Frutos, A Jiménez, R García, J Gómez, R Andrés, LM de la Calle-Lombana, L Rico
Journal of the American College of Cardiology 2002
Inducible nitric oxide synthase is expressed in synovial fluid granulocytes
J Cedergren, T Forslund, T Sundqvist, T Skogh
Clinical & Experimental Immunology 2002
Nitric oxide released from iNOS in polymorphonuclear leukocytes makes them deformable in an autocrine manner
H Kobayashi, T Cui, M Ando, R Hataishi, T Imasaki, H Mitsufuji, I Hayashi, T Tomita
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B Zhang, GL Cao, A Cross, JB Domachowske, GM Rosen
Nitric Oxide 2002
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RK Johansson, M Poljakovic, KE Andersson, K Persson
The Journal of Urology 2002
Expression of Nitric Oxide Synthase in Bladder Smooth Muscle Cells: Regulation by Cytokines and L-Arginine
R Johansson, M Poljakovic, KE Andersson, K Persson
The Journal of Urology 2002
Analysis of the mechanisms of human cytotoxic T lymphocyte response inhibition by NO
S Blesson, J Thiery, C Gaudin, R Stancou, JP Kolb, JL Moreau, J Theze, F Mami-Chouaib, S Chouaib
International Immunology 2002
Lung Biology in Health and Disease
M Wills-Karp
Lung Biology in Health and Disease: Experimental Models and Therapeutic Potential 2002
Production of nitric oxide by mussel (Mytilus galloprovincialis) hemocytes and effect of exogenous nitric oxide on phagocytic functions
C Tafalla, B Novoa, A Figueras
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2002

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