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Abstract

The identification of human inflammatory cells that express inducible nitric oxide synthase and the clarification of the role of inducible nitric oxide synthase in human infectious or inflammatory processes have been elusive. In neutrophil-enriched fractions from urine, we demonstrate a 43-fold increase in nitric oxide synthase activity in patients with urinary tract infections compared with that in neutrophil-enriched fractions from noninfected controls. Partially purified inducible nitric oxide synthase is primarily membrane associated, calcium independent, and inhibited by arginine analogues with a rank order consistent with that of purified human inducible nitric oxide synthase. Molecular, biochemical, and immunocytochemical evidence unequivocally identifies inducible nitric oxide synthase as the major nitric oxide synthase isoform found in neutrophils isolated from urine during urinary tract infections. Elevated inducible nitric oxide synthase activity and elevated nitric oxide synthase protein measured in patients with urinary tract infections and treated with antibiotics does not decrease until 6-10 d of antibiotic treatment. The extended elevation of neutrophil inducible nitric oxide synthase during urinary tract infections may have both antimicrobial and proinflammatory functions.

Authors

M A Wheeler, S D Smith, G García-Cardeña, C F Nathan, R M Weiss, W C Sessa

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Year: 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 Total
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Citations to this article in year 2001 (16)

Title and authors Publication Year
Handbook of Antioxidants
K Meissner, W Siems, O Sommerburg, M Leichsenring
Handbook of Antioxidants 2001
Effect of adhesion on inducible nitric oxide synthase (iNOS) production in purified human neutrophils
JL Webb, JM Polak, TJ Evans
Clinical & Experimental Immunology 2001
Escherichia coli-induced inducible nitric oxide synthase and cyclooxygenase expression in the mouse bladder and kidney
M Poljakovic, ML Svensson, C Svanborg, K Johansson, B Larsson, K Persson
Kidney International 2001
Nitric Oxide Synthase Activity in Neutrophils From Patients With Localized Aggressive Periodontitis
K Shibata, ML Warbington, BJ Gordon, H Kurihara, TE van Dyke
Journal of Periodontology 2001
Antineutrophil cytoplasm antibody-induced neutrophil nitric oxide production is nitric oxide synthase independent
WY Tse, J Williams, A Pall, M Wilkes, CO Savage, D Adu
Kidney International 2001
Cyclooxygenase-2 protein and prostaglandin E2 production are up-regulated in a rat bladder inflammation model
MA Wheeler, JH Yoon, LE Olsson, RM Weiss
European Journal of Pharmacology 2001
Effect of adhesion on inducible nitric oxide synthase (iNOS) production in purified human neutrophils
JL Webb, JM Polak, TJ Evans
Clinical & Experimental Immunology 2001
Bactericidal activity of human eosinophilic granulocytes against Escherichia coli
T Persson, P Andersson, M Bodelsson, M Laurell, J Malm, A Egesten
Infection and immunity 2001
Dexamethasone impairs pulmonary defence against Pseudomonas aeruginosa through suppressing iNOS gene expression and peroxynitrite production in mice
S Satoh, K Oishi, A Iwagaki, M Senba, T Akaike, M Akiyama, N Mukaida, KM Atsushima, T Nagatake
Clinical & Experimental Immunology 2001
Effects of the synthetic selectin inhibitor TBC1269 on tissue damage during acute Mannheimia haemolytica-induced pneumonia in neonatal calves
ZA Radi, JM Caverly, RA Dixon, KA Brogden, MR Ackermann
American Journal of Veterinary Research 2001
Cytotoxic effects of peroxynitrite, polymorphonuclear neutrophils, free-radical scavengers, inhibitors of myeloperoxidase, and inhibitors of nitric oxide synthase on bovine mammary secretory epithelial cells
TK Ledbetter, MJ Paape, LW Douglass
American Journal of Veterinary Research 2001
Dexamethasone impairs pulmonary defence against Pseudomonas aeruginosa through suppressing iNOS gene expression and peroxynitrite production in mice
S Satoh, K Oishi, A Iwagaki, M Senba, T Akaike, M Akiyama, N Mukaida, KM Atsushima, T Nagatake
Clinical & Experimental Immunology 2001
INDUCIBLE NITRIC OXIDE SYNTHASE IN THE BLADDER OF SPINAL CORD INJURED PATIENTS WITH A CHRONIC INDWELLING URINARY CATHETER
BM Wall, RR Dmochowski, M Malecha, T Mangold, MA Cooke
The Journal of Urology 2001
Anti-inflammatory effects of macrolide antibiotics
O Čulić, V Eraković, MJ Parnham
European Journal of Pharmacology 2001
INDUCIBLE NITRIC OXIDE SYNTHASE IN THE BLADDER OF SPINAL CORD INJURED PATIENTS WITH A CHRONIC INDWELLING URINARY CATHETER
BM Wall, RR Dmochowski, M Malecha, T Mangold, MA Cooke
The Journal of Urology 2001
The physiological role and pharmacological potential of nitric oxide in neutrophil activation
R Armstrong
International Immunopharmacology 2001

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