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Abstract

The identification of human inflammatory cells that express inducible nitric oxide synthase and the clarification of the role of inducible nitric oxide synthase in human infectious or inflammatory processes have been elusive. In neutrophil-enriched fractions from urine, we demonstrate a 43-fold increase in nitric oxide synthase activity in patients with urinary tract infections compared with that in neutrophil-enriched fractions from noninfected controls. Partially purified inducible nitric oxide synthase is primarily membrane associated, calcium independent, and inhibited by arginine analogues with a rank order consistent with that of purified human inducible nitric oxide synthase. Molecular, biochemical, and immunocytochemical evidence unequivocally identifies inducible nitric oxide synthase as the major nitric oxide synthase isoform found in neutrophils isolated from urine during urinary tract infections. Elevated inducible nitric oxide synthase activity and elevated nitric oxide synthase protein measured in patients with urinary tract infections and treated with antibiotics does not decrease until 6-10 d of antibiotic treatment. The extended elevation of neutrophil inducible nitric oxide synthase during urinary tract infections may have both antimicrobial and proinflammatory functions.

Authors

M A Wheeler, S D Smith, G García-Cardeña, C F Nathan, R M Weiss, W C Sessa

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Total citations by year

Year: 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 Total
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Citation information

Citations to this article in year 1998 (15)

Title and authors Publication Year
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Inducible nitric oxide synthase in human diseases
KD Kröncke, K Fehsel, V Kolb-Bachofen
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Cloning and characterization of human inducible nitric oxide synthase splice variants: a domain, encoded by exons 8 and 9, is critical for dimerization
NT Eissa, JW Yuan, CM Haggerty, EK Choo, CD Palmer, J Moss
Proceedings of the National Academy of Sciences of the United States of America 1998
Interleukin-15 activates proinflammatory and antimicrobial functions in polymorphonuclear cells
T Musso, L Calosso, M Zucca, M Millesimo, M Puliti, S Bulfone-Paus, C Merlino, D Savoia, R Cavallo, AN Ponzi, R Badolato
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Kidney International 1998
Stimulus-dependent transduction mechanisms for nitric oxide release in human polymorphonuclear neutrophil leukocytes
G Lärfars, H Gyllenhammar
Journal of Laboratory and Clinical Medicine 1998
Peroxynitrite production by human neutrophils, monocytes and lymphocytes challenged with lipopolysaccharide
C Gagnon, FA Leblond, JG Filep
FEBS Letters 1998
Contribution of NO synthases to neutrophil infiltration in the gastric mucosal lesions in rats with water immersion restraint stress
K Nishida, Y Ohta, I Ishiguro
FEBS Letters 1998
Myeloperoxidase and horseradish peroxidase catalyze tyrosine nitration in proteins from nitrite and hydrogen peroxide
JB Sampson, Y Ye, H Rosen, JS Beckman
Archives of Biochemistry and Biophysics 1998
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NITRIC OXIDE IN THE FAILING MYOCARDIUM
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