Article tools
Author information
Published in Volume 98, Issue 2 (July 15,1996)
J. Clin. Invest. 98(2): 467-473 (1996). doi:10.1172/JCI118813.
Copyright © 1996, The American Society for Clinical Investigation

Research Article

Regulation of human heart contractility by essential myosin light chain isoforms.

M Morano, U Zacharzowski, M Maier, P E Lange, V Alexi-Meskishvili, H Haase and I Morano

Max-Delbrück-Center for Molecular Medicine, Berlin, Germany.

Published July 15, 1996

Most of the patients with congenital heart diseases express the atrial myosin light chain 1 (ALC-1) in the right ventricle. We investigated the functional consequences of ALC-1 expression on the myosin cycling kinetics in the intact sarcomeric structure using multicellular demembranated fibers ("skinned fibers") from the right ventricular infundibulum of patients with Tetralogy of Fallot (TOF), double outlet right ventricle (DORV), and infundibular pulmonary stenosis (IPS), Force-velocity relation was analyzed by the constant-load technique at maximal Ca2+ activation (pCa 4.5). Half-time of tension development (t1/2) was investigated by monitoring contraction initiation upon photolytic release of ATP from caged-ATP in rigor. The patients investigated here expressed between 0 and 27% ALC-1. There was a statistically significant correlation between ALC-l and maximal shortening velocity (Vmax) which rose 1.87-fold from 1.2 muscle length per second (ML/s) to 2.25 ML/s in a normal (0% ALC-1) and diseased (19.9% ALC-1) ventricle. Half-time of tension development decreased 1.85-fold with increasing ALC-1 expression (t1/2) was 0.252 s and 0.136 s at 2 and 18.4% ALC-1, respectively). We conclude that the expression of ALC-1 in the human heart modulates cross-bridge cycling kinetics accelerating shortening velocity and isometric tension production.

This article Copyright © 1996, The American Society for Clinical Investigation.

Articles that cite this article:

Fine-tuning of cross-bridge kinetics in cardiac muscle of rat and mouse by myosin light chain isoforms
Oleg Andruchov, Olena Andruchova, Stefan Galler
Pflugers Arch 452(6):667. doi:10.1007/s00424-006-0080-7 [CrossRef]

Remodeling of the hypertrophied human myocardium by cardiac bHLH transcription factors
Oliver Ritter, Hannelore Haase, Hagen Dieter Schulte, Peter E. Lange, Ingo Morano
J Cell Biochem 74(4):551. doi:10.1002/(SICI)1097-4644(19990915)74:4<551::AID-JCB5>3.0.CO;2-9 [CrossRef]

Depletion of zebrafish essential and regulatory myosin light chains reduces cardiac function through distinct mechanisms
Z. Chen, W. Huang, T. Dahme, W. Rottbauer, M. J. Ackerman, X. Xu
Cardiovasc Res 79(1):97. doi:10.1093/cvr/cvn073 [CrossRef]

The human β-myosin heavy chain gene: Sequence diversity and functional characteristics of the protein
Birgit Wendel, Richard Reinhard, Ute Wachtendorf, Udo B. Zacharzowsky, Karl J. Osterziel, Hagen D. Schulte, Hannelore Haase, Margret R. Hoehe, Ingo Morano
J Cell Biochem 79(4):566. doi:10.1002/1097-4644(20001215)79:4<566::AID-JCB50>3.0.CO;2-E [CrossRef]

Plakoglobin is essential for myocardial compliance but dispensable for myofibril insertion into adherens junctions
C.M. Isac, P. Ruiz, B. Pfitzmaier, H. Haase, W. Birchmeier, I. Morano
J Cell Biochem 72(1):8. doi:10.1002/(SICI)1097-4644(19990101)72:1<8::AID-JCB2>3.0.CO;2-A [CrossRef]

Role of endogenous antisense RNA in cardiac gene regulation
Hans Peter Luther
J Mol Med 83(1):26. doi:10.1007/s00109-004-0613-5 [CrossRef]

ROLE OF CARDIAC NEURAL CREST CELLS IN CARDIOVASCULAR DEVELOPMENT
Tony L. Creazzo, Robert E. Godt, Linda Leatherbury, Simon J. Conway, Margaret L. Kirby
Annu Rev Physiol 60(1):267. doi:10.1146/annurev.physiol.60.1.267 [CrossRef]

The molecular phenotype of human cardiac myosin associated with hypertrophic obstructive cardiomyopathy
A. M. Jacques, N. Briceno, A. E. Messer, C. E. Gallon, S. Jalilzadeh, E. Garcia, G. Kikonda-kanda, J. Goddard, S. E. Harding, H. Watkins
Cardiovasc Res 79(3):481. doi:10.1093/cvr/cvn094 [CrossRef]

Thick Filament Proteins and Performance in Human Heart Failure
Bradley M. Palmer
Heart Fail Rev 10(3):187. doi:10.1007/s10741-005-5249-1 [CrossRef]

Kinetics and Energetics of The Crossbridge Cycle
David W. Maughan
Heart Fail Rev 10(3):175. doi:10.1007/s10741-005-5248-2 [CrossRef]

In vitro motility assay of atrial and ventricular myosin from pig
Christina Svensson, Ingo Morano, Anders Arner
J Cell Biochem 67(2):241. doi:10.1002/(SICI)1097-4644(19971101)67:2<241::AID-JCB9>3.0.CO;2-X [CrossRef]

Preparation of Monoclonal Antibodies against Human Ventricular Myosin Light Chain 1 (HVMLC1) for Functional Studies
Zhen-Yan Fu, Bao-Tong Xie, Yi-Tong Ma, Zu-Xun Gong
Acta Biochim Biophys Sinica 38(9):625. doi:10.1111/j.1745-7270.2006.00203.x [CrossRef]

Tension generation and relaxation in single myofibrils from human atrial and ventricular myocardium
Nicoletta Piroddi, Alexandra Belus, Beatrice Scellini, Chiara Tesi, Gabriele Giunti, Elisabetta Cerbai, Alessandro Mugelli, Corrado Poggesi
Pflugers Arch 454(1):63. doi:10.1007/s00424-006-0181-3 [CrossRef]

Molecular Genetic Basis of Hypertrophic Cardiomyopathy:.
ALI J. Marian, ROBERT Roberts
J Cardiovasc Electrophysiol 9(1):88. doi:10.1111/j.1540-8167.1998.tb00871.x [CrossRef]