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Natural killer type 2 bias in remission of multiple sclerosis
Kazuya Takahashi, Sachiko Miyake, Takayuki Kondo, Keiji Terao, Megumi Hatakenaka, Shuji Hashimoto, Takashi Yamamura
Published in Volume 107, Issue 5
J Clin Invest. 2001; 107(5):0–0 doi:10.1172/JCI11819
This article Copyright © 2001, The American Society for Clinical Investigation
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Figure 4

Intracellular staining of IFN-γ and IL-4 in T cells stimulated with PMA/ionomycin. Freshly isolated T cells (8 × 105/ml) were mixed with either NK1 or NK2 cells (2 × 105/ml) that had been generated from the same donor. The T/NK cell mixtures or T cells without NK cells were stimulated with PMA/ionomycin at 37°C for 6 hours, and the stimulated cells were stained with anti–CD3-PerCP and anti–CD56-FITC and then fixed and permeabilized. The cells were stained with either anti–IFN-γ-PE or anti–IL-4-PE, and CD56– lymphocytes were analyzed for expression of IFN-γ or IL-4. The percent values shown in the upper right quadrant denote the percentages of IFN-γ+ or IL-4+ cells among CD56–CD3+ T cells.

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