P Fanen, S Guidoux, C O Sarde, J L Mandel, M Goossens, P Aubourg
J Clin Invest.
1994;
94(2):516–520
doi:10.1172/JCI117363
This article Copyright © 1994, The American Society for Clinical Investigation
Abstract
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T
he recently identified adrenoleukodystrophy (ALD) gene is predicted to encode a peroxisomal protein of 745 amino acids that includes one domain for ATP-binding, termed nucleotide-binding fold (NBF). To determine whether mutations occur in the putative NBF of ALD protein, we analyzed by denaturing gradient gel electrophoresis (DGGE) exon 6 and 8 that encode most part of this domain in 50 ALD patients. Four amino acid substitutions, three frameshift mutations leading to premature termination signal, and a splicing mutation were identified. These amino acid substitutions occurred at residues highly conserved in other ATP-binding cassette (ABC) proteins. In addition, a nonsense mutation was detected in exon 4.
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