J Clin Invest.
Preclinical administration of anti–VLA-4 blocks infiltration of CD4+ T cells into the CNS. Lower thoracic spinal cord, cerebellar, and brainstem tissue sections from representative animals of the treatment groups described in Figure 1a were examined for infiltration of inflammatory cells on day 13 after immunization. Three treatments of control Ab or anti–VLA-4 had been given by this time point. DAPI, in blue, stains all cells; CD4+ cells are marked in red. (a) Spinal cord CD4+ infiltrate in a naive animal. (b) Spinal cord CD4+ infiltrate in a control Ig-treated animal. (c) Spinal cord CD4+ infiltrate in an anti–VLA-4–treated animal. All sections are 6 μm thick. ×100.