JCI - Antigen-pulsed dendritic cells expressing macrophage-derived chemokine elicit Th2 responses and promote specific humoral immunity

Antigen-pulsed dendritic cells expressing macrophage-derived chemokine elicit Th2 responses and promote specific humoral immunity
J. Clin. Invest. 108:6 doi:10.1172/JCI11564 [Go to this article.]

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Figure 5

Immunization with syngenic AdMDC-modified DCs pulsed with P. aeruginosa protects mice against lethal P. aeruginosa respiratory challenge. Mice were immunized intravenously with AdMDC-modified DCs pulsed with heat-killed P. aeruginosa strain PAO1 (DC/MDC + PA), AdNull-modified DCs pulsed with heat-killed P. aeruginosa strain PAO1 (DC/AdNull + PA), or AdMDC-modified DCs alone (DC/MDC) at 10⁵ DCs per mouse. Controls included naive mice without any immunization (no immunization). After 3 weeks, 2 × 10⁵ CFU P. aeruginosa (PAO1) enmeshed in agar beads was administered intratracheally. Survival was recorded as the percentage of surviving animals (n = 10 mice per group). (a) C57BL/6 mice. (b) BALB/c mice.