JCI - Carbon monoxide of vascular origin attenuates the sensitivity of renal arterial vessels to vasoconstrictors

Carbon monoxide of vascular origin attenuates the sensitivity of renal arterial vessels to vasoconstrictors

Jun-Ichi Kaide, Fan Zhang, Yuan Wei, Houli Jiang, Changhua Yu, WenHui Wang, Michael Balazy, Nader G. Abraham, Alberto Nasjletti

Published in Volume 107, Issue 9

J Clin Invest. 2001; 107(9):1163–1171 doi:10.1172/JCI11218

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Figure 3

Effect of exogenous CO on phenylephrine-induced contraction of rat renal interlobar artery rings maintained in organ culture for 18 hours prior to experimentation in media containing both HO-1 AS-ODN (40 μg/ml) and HO-2 AS-ODN (40 μg/ml) (a) or the corresponding scrambled oligodeoxynucleotides (HO-1 S-ODN and HO-2 S-ODN, both at 40 μg/ml) (b). (a) HO-1 AS-ODN + HO-2 AS-ODN without CO (EC₅₀, 0.16 ± 0.02 μmol/l; R_max, 4.24 ± 0.37 mN/mm; n = 9), with 0.1 μmol/l CO (EC₅₀, 0.53 ± 0.06^A μmol/l; R_max, 4.14 ± 0.29 mN/mm; n = 9). and with 1.0 μmol/l CO (EC₅₀, 0.84 ± 0.10^A μmol/l; R_max, 4.08 ± 0.33 mN/mm; n = 9). (b) HO-1 S-ODN + HO-2 S-ODN without CO (EC₅₀, 0.52 ± 0.06 μmol/l; R_max, 4.31 ± 0.16 mN/mm; n = 8), with 0.1 μmol/l CO (EC₅₀, 0.49 ± 0.07 μmol/l; R_max, 4.27 ± 0.19 mN/mm; n = 8), and with 1.0 μmol/l CO (EC₅₀, 0.47 ± 0.06 μmol/l; R_max, 4.29 ± 0.18 mN/mm; n = 8). L-NAME (1 mmol/l) was included in the buffer used in contractility studies. Results are mean ± SEM. ^AP < 0.05 relative to corresponding data in vessels not exposed to exogenous CO.