JCI - Carbon monoxide of vascular origin attenuates the sensitivity of renal arterial vessels to vasoconstrictors

Carbon monoxide of vascular origin attenuates the sensitivity of renal arterial vessels to vasoconstrictors

Jun-Ichi Kaide, Fan Zhang, Yuan Wei, Houli Jiang, Changhua Yu, WenHui Wang, Michael Balazy, Nader G. Abraham, Alberto Nasjletti

Published in Volume 107, Issue 9

J Clin Invest. 2001; 107(9):1163–1171 doi:10.1172/JCI11218

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Figure 2

Concentration-response curves to phenylephrine in rat renal interlobar artery rings maintained in organ culture for 18 hours prior to testing in media containing HO-1 AS-ODN (40 μg/ml; EC₅₀, 0.45 ± 0.15 μmol/l; R_max, 4.33 ± 0.28 mN/mm; n = 8) or the corresponding scrambled oligodeoxynucleotide (HO-1 S-ODN, 40 μg/ml; EC₅₀, 0.52 ± 0.05 μmol/l; R_max, 4.41 ± 0.24 mN/mm; n = 8) (left panel), HO-2 AS-ODN (40 μg/ml; EC₅₀, 0.13 ± 0.02^A μmol/l; R_max, 4.26 ± 0.25 mN/mm; n = 16) or the corresponding scrambled oligodeoxynucleotides (HO-2 S-ODN, 40 μg/ml; EC₅₀, 0.46 ± 0.04 μmol/l; R_max, 4.18 ± 0.17 mN/mm; n = 17) (middle panel), both HO-1 AS-ODN and HO-2 AS-ODN (EC₅₀, 0.14 ± 0.02^A μmol/l; R_max, 4.28 ± 0.20 mN/mm; n = 17) or HO-1 S-ODN and HO-2 S-ODN in combination (EC₅₀, 0.52 ± 0.06 μmol/l; R_max, 4.46 ± 0.16 mN/mm; n = 8) (right panel). L-NAME (1 mmol/l) was included in the buffer used in contractility studies. Results are mean ± SEM. ^AP < 0.05 relative to corresponding data in vessels treated with scrambled oligodeoxynucleotides.