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Abstract

Peripheral blood lymphocytes and splenocytes of patients with autoimmune disease were used to prepare human-human hybridomas that produce autoantibodies. Because exogenous immunization was not used, the hybridoma antibodies were derived from B cells that spontaneously produced autoantibodies. 108 hybrids grew from 4,254 wells (2.5%). Optimal conditions for obtaining hybridomas with the GM 4672 myeloma line included initial growth in 2-ml wells, the use of 44% polyethylene glycol, a mononuclear cell/GM 4672 cell ratio 5:1, and prior stimulation of the B lymphocytes with pokeweed mitogen. Hybridoma supernatants had activity against ssDNA, platelets, and erythrocytes. The results demonstrate the feasibility of producing human-human hybridomas from lymphocytes of patients with various autoimmune diseases.

Authors

Y Shoenfeld ... B D Stollar, R S Schwartz

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Citations to this article in year 1997 (6)

Title and authors Publication Year
Natural Autoantibodies, Tolerance, and Autoimmunity
G Dighiero
Annals of the New York Academy of Sciences 1997
Modulation of murine systemic lupus erythematosus with peptides based on complementarity determining regions of a pathogenic anti-DNA monoclonal antibody
A Waisman, PJ Ruiz, E Israeli, E Eilat, S Könen-Waisman, H Zinger, M Dayan, E Mozes
Proceedings of the National Academy of Sciences of the United States of America 1997
The Role of Autoreactivity in B Cell Selection
BD Stollar, JM Lecerf, Y Hirabayashi
Annals of the New York Academy of Sciences 1997
Modulation of murine systemic lupus erythematosus with peptides based on complementarity determining regions of a pathogenic anti-DNA monoclonal antibody
A Waisman, PJ Ruiz, E Israeli, E Eilat, S Konen-Waisman, H Zinger, M Dayan, E Mozes
Proceedings of the National Academy of Sciences 1997
Immunoregulation in Health and Disease
L Tomašević, E Maggi, S Romagnani
Immunoregulation in Health and Disease 1997
Idiotypes in Medicine: Autoimmunity, Infection and Cancer
TJ Kunicki, DJ Nugent
Idiotypes in Medicine: Autoimmunity, Infection and Cancer 1997

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