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Peripheral blood lymphocytes and splenocytes of patients with autoimmune disease were used to prepare human-human hybridomas that produce autoantibodies. Because exogenous immunization was not used, the hybridoma antibodies were derived from B cells that spontaneously produced autoantibodies. 108 hybrids grew from 4,254 wells (2.5%). Optimal conditions for obtaining hybridomas with the GM 4672 myeloma line included initial growth in 2-ml wells, the use of 44% polyethylene glycol, a mononuclear cell/GM 4672 cell ratio 5:1, and prior stimulation of the B lymphocytes with pokeweed mitogen. Hybridoma supernatants had activity against ssDNA, platelets, and erythrocytes. The results demonstrate the feasibility of producing human-human hybridomas from lymphocytes of patients with various autoimmune diseases.


Y Shoenfeld ... B D Stollar, R S Schwartz


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Citations to this article in year 1989 (16)

Title and authors Publication Year
Molecular and genetic characteristics of pathogenic autoantibodies
F Tron, JF Bach
Journal of Autoimmunity 1989
Binding of Monoclonal Anti-DNA and Anti-TB Glycolipids to Brain Tissue
H Amital-Teplizki, I Avinoach, AR Coates, O Kooperman, M Blank, Y Shoenfeld
Autoimmunity 1989
Microplate elsa for detection of antibodies to DNA in patients with systemic lupus erythematosus: Specificity and correlation with farr radioimmunoassay
M Sutjita, A Hohmann, ML Boey, J Bradley
Journal of Clinical Laboratory Analysis 1989
Generation of monoclonal anti-dermatan sulphate antibodies cross-reacting to calf thymus DNA
Clinical & Experimental Immunology 1989
Serological abnormalities, including common idiotype PR4, in families with rheumatoid arthritis
P Youinou, W Williams, PL Goff, N Tuaillon, J Jouquan, S Muller, DA Isenberg
Annals of the rheumatic diseases 1989
Autoantibody production by patients infected with Leishmania
S Argov, CL Jaffe, M Krupp, H Slor, Y Shoenfeld
Clinical & Experimental Immunology 1989
Natural autoantibodies in the serum of healthy women--a five-year follow-up
O Yadin, B Sarov, L Naggan, H Slor, Y Shoenfeld
Clinical & Experimental Immunology 1989
A common anti-cardiolipin antibody idiotype in autoimmune disease: identification using a mouse monoclonal antibody directed against a naturally-occurring anti-phospholipid antibody
M Sutjita, A Hohmann, R Comacchio, ML Boey, J Bradley
Clinical & Experimental Immunology 1989
Serum monoclonal antibodies derived from patients with multiple myeloma react with mycobacterial phosphoinositides and nuclear antigens
D Buskila, M Abu-Shakra, H Amital-Teplizki, AR Coates, M Krupp, S Sukenik, Y Shoenfeld
Clinical & Experimental Immunology 1989
Establishment of an anti-A human monoclonal antibody from a blood group A lung cancer patient: evidence for the occurrence of autoimmune response to difucosylated type-2 chain A
H Inoue, S Hirohashi, Y Shimosato, M Enjoji, H Clausen, SI Hakomori
European Journal of Immunology 1989
Advances in Clinical Chemistry
R Reckel
Advances in Clinical Chemistry 1989
Reproductive failure because of autoantibodies: Unexplained infertility and pregnancy wastage
N Gleicher, A El-Roeiy, E Confino, J Friberg
American Journal of Obstetrics and Gynecology 1989
The role of the human anti-DNA idiotype in autoimmunity
Y Shoenfeld, HA Teplizki, S Mendlovic, M Blank, E Mozes, DA Isenberg
Clinical Immunology and Immunopathology 1989
Productive Ascites Growth of Heterohybridomas between Epstein-Barr Virus-Transformed Human B Cells and Murine P3×63Ag8.653 Myeloma Cells
M Matsumoto, K Mochizuki, Y Kobayashi
Microbiology and Immunology 1989
Sequential anti-idiotypes define reciprocal idiotopes on the same anti-DNA antibody
Y Naparstek, C Mackworth-Young, L Breitman, RS Schwartz
Clinical Immunology and Immunopathology 1989
Immunointervention in Autoimmune Diseases
M Blank, S Mendelovic, E Mozest, Y Shoenfeld
Immunointervention in Autoimmune Diseases 1989