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Abstract

Peripheral blood lymphocytes and splenocytes of patients with autoimmune disease were used to prepare human-human hybridomas that produce autoantibodies. Because exogenous immunization was not used, the hybridoma antibodies were derived from B cells that spontaneously produced autoantibodies. 108 hybrids grew from 4,254 wells (2.5%). Optimal conditions for obtaining hybridomas with the GM 4672 myeloma line included initial growth in 2-ml wells, the use of 44% polyethylene glycol, a mononuclear cell/GM 4672 cell ratio 5:1, and prior stimulation of the B lymphocytes with pokeweed mitogen. Hybridoma supernatants had activity against ssDNA, platelets, and erythrocytes. The results demonstrate the feasibility of producing human-human hybridomas from lymphocytes of patients with various autoimmune diseases.

Authors

Y Shoenfeld ... B D Stollar, R S Schwartz

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Citation information

Citations to this article in year 1987 (17)

Title and authors Publication Year
The Immunogenicity of Platelet Membrane Glycoproteins
TJ Kunicki, K Furihata, B Bull, DJ Nugent
Transfusion Medicine Reviews 1987
Purification of antigens and monoclonal antibodies
RN Maini, D Williams
Clinical Rheumatology 1987
Human monoclonal antibodies as cellular and molecular probes: a review
MJ O'Hare, CY Yiu
Molecular and Cellular Probes 1987
DNA antibody idiotypes: A review of their genetic, clinical, and immunopathologic features
Y Shoenfeld, DA Isenberg
Seminars in Arthritis and Rheumatism 1987
Platelet antibodies in systemic lupus erythematosus
C Kaplan, P Champeix, D Blanchard, JY Muller, JP Cartron
British Journal of Haematology 1987
Description and partial characterization of a nucleolar RNA-associated autoantigen defined by a human monoclonal antibody
N Chiorazzi, WH Reeves
Journal of Experimental Medicine 1987
A common anti-DNA idiotype and other autoantibodies in sera of offspring of mothers with systemic lupus erythematosus
A el-Roeiy, N Gleicher, D Isenberg, RC Kennedy, Y Shoenfeld
Clinical & Experimental Immunology 1987
Idiotypic markers of polyclonal B cell activation. Public idiotypes shared by monoclonal antibodies derived from patients with systemic lupus erythematosus or leprosy
C Mackworth-Young, J Sabbaga, RS Schwartz
Journal of Clinical Investigation 1987
Parallel sets of autoantibodies in MRL-lpr/lpr mice. An anti-DNA, anti-SmRNP, anti-gp70 network
P Migliorini, B Ardman, J Kaburaki, RS Schwartz
Journal of Experimental Medicine 1987
Estimation of Heterokaryon Formation and Hybridoma Growth in Murine and Human Cell Fusions
SS Alkan, F Mestel, J Jiricka, K Blaser
Hybridoma 1987
Anti-platelet and Anti-DNA IgM in waldenström macroglobulinemia and ITP
L Vartlcovski, AI Pick, A Schattner, Y Shoenfeld
American Journal of Hematology 1987
Human monoclonal antibodies derived from lymph nodes of a patient with breast carcinoma react with MuMTV polypeptides
Y Shoenfeld, A Hizi, R Tal, NI Smorodinsky, G Lavie, C Mor, S Schteren, Z Mammon, J Pinkhas, T Keydar
Cancer 1987
Specificity of anti-polynucleotide monoclonal antibodies from human-human hybridomas
S Hashizume, H Murakami, M Kamei, S Hirose, T Shirai, K Yamada, H Omura
In Vitro Cellular & Developmental Biology 1987
Immunization of normal human splenocytes in vitro to produce human monoclonal antibodies to cellular antigens
CM Hulette, RB Effros, RL Walford
Tissue Antigens 1987
Human monoclonal antibody production
K James, GT Bell
Journal of Immunological Methods 1987
Polyspecific reactivity of a murine monoclonal antibody that binds to nuclear matrix-associated, chromatin-bound autoantigens
AJ Laster, DS Pisetsky, BF Haynes
Clinical Immunology and Immunopathology 1987
Methods in Enzymology
SL Spitalnik
Methods in enzymology 1987

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