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Peripheral blood lymphocytes and splenocytes of patients with autoimmune disease were used to prepare human-human hybridomas that produce autoantibodies. Because exogenous immunization was not used, the hybridoma antibodies were derived from B cells that spontaneously produced autoantibodies. 108 hybrids grew from 4,254 wells (2.5%). Optimal conditions for obtaining hybridomas with the GM 4672 myeloma line included initial growth in 2-ml wells, the use of 44% polyethylene glycol, a mononuclear cell/GM 4672 cell ratio 5:1, and prior stimulation of the B lymphocytes with pokeweed mitogen. Hybridoma supernatants had activity against ssDNA, platelets, and erythrocytes. The results demonstrate the feasibility of producing human-human hybridomas from lymphocytes of patients with various autoimmune diseases.


Y Shoenfeld ... B D Stollar, R S Schwartz


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Citations to this article in year 1986 (17)

Title and authors Publication Year
Human Monoclonal Antibodies as Probes to Study Autoimmune and Allergic Disorders
N Chiorazzi
Bio/Technology 1986
Antibodies to DNA. A perspective
W Emlen, DS Pisetsky, RP Taylor
Arthritis & Rheumatism 1986
Monoclonal Anti-DNA Antibodies The Targets and Origins of SLE Autoantibodies
BD Stollar, RS Schwartz
Annals of the New York Academy of Sciences 1986
A monoclonal antibody reactive with a subset of human plasma cells
NJ Gonchoroff, JA Katzmann, JP Garton, GJ Ruiz-Argüelles, CR Eilers, PR Greipp, RA Kyle
British Journal of Haematology 1986
A human monoclonal macroglobulin with specificity for alpha(2----8)-linked poly-N-acetyl neuraminic acid, the capsular polysaccharide of group B meningococci and Escherichia coli K1, which crossreacts with polynucleotides and with denatured DNA
EA Kabat, KG Nickerson, J Liao, L Grossbard, EF Osserman, E Glickman, L Chess, JB Robbins, R Schneerson, YH Yang
Journal of Experimental Medicine 1986
A study of anti-poly (ADP-ribose) antibodies and an anti-DNA antibody idiotype and other immunological abnormalities in lupus family members
C Dudeney, Y Shoenfeld, J Rauch, M Jones, CM Young, M Tavassoli, S Shall, DA Isenberg
Annals of the rheumatic diseases 1986
Monoclonal anti-tuberculosis antibodies react with DNA, and monoclonal anti-DNA autoantibodies react with Mycobacterium tuberculosis
Y Shoenfeld, Y Vilner, AR Coates, J Rauch, G Lavie, D Shaul, J Pinkhas
Clinical & Experimental Immunology 1986
A single germline VH gene segment of normal A/J mice encodes autoantibodies characteristic of systemic lupus erythematosus
Y Naparstek, J André-Schwartz, T Manser, LJ Wysocki, L Breitman, BD Stollar, M Gefter, RS Schwartz
Journal of Experimental Medicine 1986
Human-human hybridomas secreting monoclonal antibodies to the Mr 195,000 Plasmodium falciparum blood stage antigen
R Schmidt-Ullrich, J Brown, H Whittle, PS Lin
Journal of Experimental Medicine 1986
Preparation and characterization of human monoclonal antibodies directed against the hepatitis B virus surface antigen
G Colucci, DS Kohtz, SD Waksal
Liver 1986
Monoclonal Antibodies to Acetylcholine Receptor Secreted by Human X Human Hybridomas Derived from Lymphocytes of a Patient with Myasthenia Gravis
DA Blair, DP Richman, CJ Taves, S Koethe
Immunological Investigations 1986
Anti-DNA antibodies and the problem of autoimmunity
RS Schwartz
Cellular Immunology 1986
In vitro stimulation prior to fusion generates antigen-binding human-human hybridomas
RL Wasserman, RD Budens, ES Thaxton
Journal of Immunological Methods 1986
Advances in Immunology
TH Lee, KF Austen
Advances in immunology 1986
Methods in Enzymology
RR Montgomery, TJ Kunicki, LM Glode
Methods in enzymology 1986
Effects of interferon-α on the expression of a lupus idiotype in normal humans
A Schattner, D Duggan, Y Naparstek, RS Schwartz
Clinical Immunology and Immunopathology 1986
The analyses of common idiotypes of human monoclonal anti-DNA autoantibodies in SLE and related conditions
Y Schoenfeld
Clinical Immunology Newsletter 1986