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Peripheral blood lymphocytes and splenocytes of patients with autoimmune disease were used to prepare human-human hybridomas that produce autoantibodies. Because exogenous immunization was not used, the hybridoma antibodies were derived from B cells that spontaneously produced autoantibodies. 108 hybrids grew from 4,254 wells (2.5%). Optimal conditions for obtaining hybridomas with the GM 4672 myeloma line included initial growth in 2-ml wells, the use of 44% polyethylene glycol, a mononuclear cell/GM 4672 cell ratio 5:1, and prior stimulation of the B lymphocytes with pokeweed mitogen. Hybridoma supernatants had activity against ssDNA, platelets, and erythrocytes. The results demonstrate the feasibility of producing human-human hybridomas from lymphocytes of patients with various autoimmune diseases.


Y Shoenfeld ... B D Stollar, R S Schwartz


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Citations to this article in year 1983 (17)

Title and authors Publication Year
The rheumatologic complications of hematologic disorders
DA Isenberg, Y Shoenfeld
Seminars in Arthritis and Rheumatism 1983
WJ Morrow, DA Isenberg, P Youinou, ML Snaith
The Lancet 1983
Monoclonal lupus autoantibody secretion by human-human hybridomas. Selection of hybrids by conventional and novel techniques
BH Littman, AV Muchmore, AD Steinberg, WC Greene
Journal of Clinical Investigation 1983
Idiotypic cross-reactions of monoclonal human lupus autoantibodies
Y Shoenfeld, DA Isenberg, J Rauch, MP Madaio, BD Stollar, RS Schwartz
Journal of Experimental Medicine 1983
Detection of cell surface and intracellular antigens by human monoclonal antibodies. Hybrid cell lines derived from lymphocytes of patients with malignant melanoma
AN Houghton, H Brooks, RJ Cote, MC Taormina, HF Oettgen, LJ Old
Journal of Experimental Medicine 1983
Construction and testing of mouse--human heteromyelomas for human monoclonal antibody production
NN Teng, KS Lam, FC Riera, HS Kaplan
Proceedings of the National Academy of Sciences of the United States of America 1983
Antibodies reactive with surface membranes of cellular elements in the blood
DB Cines
Human Pathology 1983
Monoclonal Antibodies in Clinical Immunobiology
L Olsson
Allergy 1983
Immunology: Aetiology of autoimmunity
AD Steinberg
Nature 1983
Novel Approach to Construction of Human "Myeloma Analogues" for Production of Human Monoclonal Antibodies
M Posner, S Schlossman, H Lazarus
Hybridoma 1983
Antibody producing human-human hybridomas. I. Technical aspects
L Olsson, H Kronstrøm, AC Mouzon, C Honzik, T Brodin, B Jakobsen
Journal of Immunological Methods 1983
Polyspecificity of Monoclonal Lupus Autoantibodies Produced by Human-Human Hybridomas
Y Shoenfeld, J Rauch, H Massicotte, SK Datta, J André-Schwartz, BD Stollar, RS Schwartz
New England Journal of Medicine 1983
Human Monoclonal Autoantibodies That React with Multiple Endocrine Organs
J Satoh, BS Prabhakar, MV Haspel, F Ginsberg-Fellner, AL Notkins
New England Journal of Medicine 1983
Monoclonal lupus autoantibodies
RS Schwartz
Immunology Today 1983
The production of monoclonal antibodies from human lymphocytes
D Kozbor, JC Roder
Immunology Today 1983
Monoclonal Antibodies
DS Pisetsky
Monoclonal Antibodies 1983
Monoclonal Antibodies
HC Lane, AS Fauci
Monoclonal Antibodies 1983