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Abstract

To see whether circulating granulocytes are necessary for the lung vascular reaction to endotoxin, we measured the endotoxin response in chronically instrumented sheep before and after granulocyte depletion with hydroxyurea. Granulocyte depletion did not affect the pulmonary hypertension caused by endotoxin (peak mean pulmonary artery pressures = 38 +/- 2 cm H2O before depletion and 42 +/- 2 after depletion, P = NS). The late phase increase in lung lymph flow after endotoxin was significantly lower in the granulocytopenic animals as reflected by lung lymph flow (mean steady state lymph flow before depletion = 30.6 +/- 2.0 SE ml/h; mean steady state lymph flow after granulocyte depletion = 15.4 +/- 1.0; P less than 0.01) even though late phase pulmonary vascular pressures were similar before and after granulocyte depletion. Lung lymph protein clearance (lymph flow x lymph/plasma protein concentration) was also significantly lower after granulocyte depletion (mean steady state before depletion = 2.14 +/- 1.4 SE ml/h; and after depletion = 10.4 +/- 1.0; P less than 0.01). We conclude that circulating granulocytes are necessary for the development of increased lung vascular permeability to fluid and protein following endotoxin. The pulmonary vasopressor effects of endotoxin in sheep are independent of granulocytes.

Authors

A C Heflin Jr, K L Brigham

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Citations to this article in year 2004 (4)

Title and authors Publication Year
Thalidomide reduces lipopolysaccharide/zymosan-induced acute lung injury in rats
CS Chen, WC Perng, CW Chen, KL Huang, CP Wu, MH Yen
Journal of Biomedical Science 2004
Delayed neutrophil apoptosis in sepsis is associated with maintenance of mitochondrial transmembrane potential and reduced caspase-9 activity*
R Taneja, J Parodo, SH Jia, A Kapus, OD Rotstein, JC Marshall
Critical Care Medicine 2004
Leukotriene B4 receptor (BLT-1) modulates neutrophil influx into the peritoneum but not the lung and liver during surgically induced bacterial peritonitis in mice
MJ Scott, WG Cheadle, JJ Hoth, JC Peyton, K Subbarao, WH Shao, B Haribabu
Clinical and diagnostic laboratory immunology 2004
Delayed neutrophil apoptosis in sepsis is associated with maintenance of mitochondrial transmembrane potential and reduced caspase-9 activity*:
R Taneja, J Parodo, SH Jia, A Kapus, OD Rotstein, JC Marshall
Critical Care Medicine 2004

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