J Clin Invest.
Immune responses of vaccinated mice. Splenocytes from nonimmune mice (open bars) and immunized mice (filled bars) were cocultured at 5 × 106 cells/ml with medium alone, CON A (5 μg/ml), or CW/M antigen (12.5 μg/ml). Data are mean values of four independent experiments. (a and d) Proliferation was measured as cpm of [3H]TdR incorporated into proliferating cells after 4 days of coculture. (b and c) Before infection with B. dermatitidis, IL-2 and IFN-γ were measured in supernates of splenocytes, cultured as described in the text. (e and f) After infection with B. dermatitidis, studies were performed as in b and c.