Published in Volume 64, Issue 2 (August , 1979)
J. Clin. Invest.
64(2):
381-384 (1979).
doi:10.1172/JCI109472.
Copyright © 1979, The American Society for Clinical Investigation
Research Article
Gangliosides sensitize unresponsive fibroblasts to Escherichia coli heat-labile enterotoxin.
J Moss,
S Garrison,
P H Fishman and
S H Richardson
Published
August , 1979
Chemically transformed mouse fibroblasts did not raise their cyclic AMP level in response to Escherichia coli heat-labile enterotoxin. These fibroblasts did, however, incorporate exogenous mono-, di-, and trisialogangliosides. After the uptake of monosialoganglioside galactosyl-N-acetylgalactosaminyl-[N-acetylneuraminyl]-galactosylglucosylceramide (GM1), the cells responded to E. coli heat-labile enterotoxin. The di- and trisialogangliosides were considerably less effective. GM1, the putative cholera toxin (choleragen) receptor, has been implicated previously as the receptor for E. coli heat-labile enterotoxin based on the ability of the free ganglioside to inhibit the effects of toxin. This investigation establishes that the ganglioside, when incorporated into fibroblasts, serves a functional role in mediating the responsiveness to the toxin.
Browse pages
Click on an image below to see the page. View PDF of the complete article