Published in Volume
51, Issue 7 (July 1972)
J Clin Invest. 1972;51(7):1910–1914.
doi:10.1172/JCI106994.
Copyright ©
1972, The American Society for
Clinical Investigation.
Concise Publications
The appearance of lecithin-32P, synthesized from lysolecithin-32P, in phagosomes of polymorphonuclear leukocytes
P. Elsbach, P. Patriarca, P. Pettis, T. P. Stossel, R. J. Mason and M. Vaughan
Department of Medicine, New York University School of Medicine, New York 10016The Molecular Disease Branch, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland 20014
Published July 1972
Rabbit polymorphonuclear leukocytes ingesting paraffin oil particles stabilized with albumin, converted more lysolecithin-32P (added to the medium as an albumin complex) to cellular lecithin than did control cells.
Almost all of the increment in leukocyte lecithin-32P is found in association with the isolated phagocytic vacuoles.
About half of lecithin-32P of granulocytes incubated first with lysolecithin-32P and then reincubated with paraffin particles in a nonradioactive medium is transferred from a sedimentable (presumably membrane) fraction to the phagosomes. Isolated phagosomes or granules by themselves are capable of acylating lysolecithin. The main source of lysolecithin-32P for synthesis of cellular lecithin-32P, however, appears to be extracellular rather than lysolecithin-32P within the cytoplasm or the phagocytic vacuole. We interpret our findings therefore as indicating that lecithin-32P in the phagosomes derives chiefly from the outer membrane.
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