IL-4 and RANKL induce endogenous STAT6 and NF-κB interaction. Macrophages were grown in tissue culture coverslips for 3 days. Cells were then treated with RANKL (20 minutes) and/or IL-4 (60 minutes) or left untreated, followed by fixation with 0.25% glutaraldehyde. Cells were immunostained with STAT6 (red) and p52 NF-κB (green) antibodies alone or in combination as shown. Colocalization (d, yellow) is indicated.