JCI - Induction of the chemokine stromal-derived factor-1 following DNA damage improves human stem cell function

Induction of the chemokine stromal-derived factor-1 following DNA damage improves human stem cell function

Tanya Ponomaryov, Amnon Peled, Isabelle Petit, Russell S. Taichman, Liliana Habler, Judith Sandbank, Fernando Arenzana-Seisdedos, Aude Magerus, Antonio Caruz, Nobutaka Fujii, Arnon Nagler, Meir Lahav, Martin Szyper-Kravitz, Dov Zipori, Tsvee Lapidot

Published in Volume 106, Issue 11

J Clin Invest. 2000; 106(11):1331–1339 doi:10.1172/JCI10329

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Figure 6

Increased homing of human CD45⁺ cells transplanted 48 hours after treatment with TBI, Cy, or 5-FU. (a) FACS plots, CD45⁺: CB MNC, human CB MNC before transplantation; CTRL, nontransplanted murine BM; NT, transplantation of human CB MNC into nontreated mice; Irrad. 0 h, transplanted immediately after TBI; Irrad. 48 h, transplanted 48 hours after TBI; 5-FU or Cy, transplanted 48 hours after treatment. (b) Total numbers of human CD45⁺ cells in the murine BM and spleen, 24 hours after transplantation of human CB MNC (2 × 10⁷/mouse) into nontreated (NT) or treated NOD/SCID mice. Results from two experiments, mean ± SE, are shown. Amounts of human cells were calculated for the total organ based on acquisition number of cells and cell numbers in the total organ. Numbers inside the chart represent P values calculated with Student’s t test. FL-1, anti-CD45 FITC; FL-2, internal fluorescence.