JCI - Induction of the chemokine stromal-derived factor-1 following DNA damage improves human stem cell function

Induction of the chemokine stromal-derived factor-1 following DNA damage improves human stem cell function

Tanya Ponomaryov, Amnon Peled, Isabelle Petit, Russell S. Taichman, Liliana Habler, Judith Sandbank, Fernando Arenzana-Seisdedos, Aude Magerus, Antonio Caruz, Nobutaka Fujii, Arnon Nagler, Meir Lahav, Martin Szyper-Kravitz, Dov Zipori, Tsvee Lapidot

Published in Volume 106, Issue 11

J Clin Invest. 2000; 106(11):1331–1339 doi:10.1172/JCI10329

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Figure 3

Increased levels of SDF-1 after TBI. (a) SDF-1 mRNA expression in the BM of two NOD/SCID mice per time point. (b) Migration of human CD34⁺ cells to the NOD/SCID BM CM 4, 24, and 48 hours after TBI. Spontaneous migration (CTRL) and migration to SDF-1 (SDF-1). Results are mean ± SE from seven experiments. (c) Semisolid colony assay for progenitors migrating to NOD/SCID BM CM collected before or 48 hours after TBI, without or with anti-CXCR4 Ab pretreatment. (d) Engraftment by mononuclear cells (MNC), CD34⁺ cells or sorted CD34⁺/CD38^–/low cells transplanted immediately or 48 hours after TBI. Mean ± SE from nine experiments. P values determined using student’s t test. GEMM, granulocyte erythrocyte macrophage megakeryocyte; GM, granulocytes-macrophages; BFU-E, burst forming unit erythroid.