Disruption of hyaluronan synthase-2 abrogates normal cardiac morphogenesis and hyaluronan-mediated transformation of epithelium to mesenchyme
J. Clin. Invest. Todd D. Camenisch, et al. 106:349 doi:10.1172/JCI10272 [
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Figure 3Abnormalities exhibited by
Has2–/– embryos. (
a) Yolk sac of an
Has2+/– embryo. (
b) Yolk sac from an
Has2–/– littermate. Cross-sections of the yolk sac stained with hematoxylin and eosin are shown in the inset. Note the presence of vitelline vessels (VV) containing nucleated red blood cells in the yolk sac of the
Has2+/– embryo. The endoderm and mesoderm are not fused in the
Has2–/– embryo, and the red blood cells are free within this space. (
c and
d) Representative wild-type and
Has2–/– embryos at E9.5. Note the diminished size, the bloodless heart, and distorted somites of the
Has2–/– embryo. (
e and
f) E9.5 wild-type and
Has2–/– embryos stained for the endothelial marker PECAM. Note the absence of an organized vascular network expressing PECAM in the
Has2–/– embryo. P, pericardium; E, endoderm; M, mesoderm; OpP, optic placode; OtP, otic placode; first and second pharyngeal pouches are numbered. Bars in
c–
f = 500 μm.
