Treatment of murine lupus with cDNA encoding IFN-γR/Fc
J. Clin. Invest. Brian R. Lawson, et al. 106:207 doi:10.1172/JCI10167 [Go to this article.]

Figure 6
IgG deposits and expression of MHC class II, ICAM-1, and MCP-1 proteins in kidney tissues from MRL-Fas^lpr mice treated from 1 month of age with blank (VR1255) or active (VR1255-IFNγR/Fc) plasmid with electroporation. Active plasmid–treated mice had substantially reduced glomerular IgG deposits, as well as decreased levels of MHC class II, ICAM-1, and MCP-1. Similar reductions were observed in mice treated from 4 months of age with active plasmid (data not shown). IgG deposits were detected with FITC–anti-IgG antibody (×45), and the immunoperoxidase procedure for detection of MHC class II, ICAM-1, and MCP-1 was performed as described in Figure 5 (×25). Photomicrographs are representative samples from four mice per group.