Treatment of murine lupus with cDNA encoding IFN-γR/Fc
J. Clin. Invest. Brian R. Lawson, et al. 106:207 doi:10.1172/JCI10167 [
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Figure 5CD3
+ and F4/80
+ cells in kidney tissue from MRL-Fas
lpr mice treated with blank (VR1255) or active (VR1255-IFN-γR/Fc) plasmid with electroporation from 1 month of age. The numbers of T cells (CD3
+), and especially macrophages (F4/80
+), are markedly reduced in the active plasmid–treated groups. Similar reductions were observed in mice treated from 4 months of age with active plasmid (data not shown). Tissue sections were stained with either biotinylated anti-CD3 or anti-F4/80, incubated with streptavidin-horseradish peroxidase, and developed with AEC (see Methods). Photomicrographs are representative sections from four mice in each group. ×25.
